Effects of Glycerol and Fructose on Livability, Motility, and Anaerobic Gas Production of Bovine Spermatozoa

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Abstract
In vitro manometric determinations of anaerobic gas production by aliquot spermatozoan samples from 15 individual ejaculates of 5 dairy bulls indicated a slight but statistically nonsignificant inhibitory effect of glycerol. Compared to both fructose and glycerol controls, the inclusion of 1% fructose with 1.0, 3.3, or 10% glycerol caused an immediate and marked stimulation of gas production. Glycerol plus fructose maintained a greater degree of motility and livability of bovine spermatozoa at the end of 105 minutes incubation at 38[degree] C than did either fructose or glycerol alone. Under the conditions of this study, 1% glycerol plus 1% fructose was the apparent optimum for maintenance of spermatozoan motility and livability.