Enhanced and feedback-resistant γ-glutamyl kinase activity of anEscherichia colitransformant carrying a mutatedproBgene ofStreptococcus thermophilus
Open Access
- 1 January 2000
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 182 (1) , 143-147
- https://doi.org/10.1111/j.1574-6968.2000.tb08888.x
Abstract
We used a PCR-based method to generate a single base pair mutation in the proB gene of Streptococcus thermophilus, which replaced an aspartic acid with a glycine residue at position 192 of the first proline biosynthetic enzyme γ-glutamyl kinase. This was the first identified mutation in amino acid biosynthesis in S. thermophilus to our knowledge. The mutation caused an enhanced, feedback-resistant γ-glutamyl kinase activity and conferred an analogue-resistant phenotype to an Escherichia coli transformant containing the mutated gene.Keywords
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