Isolation and rapid sequence characterization of two novel bovine Β-lactoglobulins I and J
- 1 November 1996
- journal article
- Published by Springer Nature in Protein Journal
- Vol. 15 (8) , 743-750
- https://doi.org/10.1007/bf01887148
Abstract
Two novel bovineΒ-lactoglobulins I and J have been isolated from bovine milk and characterized by isoelectric focusing. Their primary structure was determined by a very rapid method consisting of a combination of Edman sequencing, mass analysis, and ladder sequencing by mass spectrometry. We found that both newΒ-lactoglobulins are of the bovineΒ-lactoglobulin B-variant type.Β-lactoglobulin I shows Gly instead of Glu at position 108, whereasΒ-lactoglobulin J shows a Pro-to-Leu exchange at position 126.Keywords
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