EVIDENCE FOR A PROPOSED INITIATION COMPLEX FOR PROTEIN SYNTHESIS IN RETICULOCYTE POLYRIBOSOME PROFILES
- 1 August 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (4) , 1206-1213
- https://doi.org/10.1073/pnas.63.4.1206
Abstract
Polysomes consisting of 40S ribosomal subunits attached to monosomes, disomes, trisomes, and tetrasomes have been isolated for the first time by the use of isokinetic sucrose density gradients. They are formed upon incubation of rabbit reticulocyte lysate in the presence of 10 mM NaF. It is proposed that these structures represent transient initiation complexes for protein synthesis.Keywords
This publication has 26 references indexed in Scilit:
- The role of ribosomal subunits and 80-S monomers in polysome formation in an ascites tumour cellBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Control of haemoglobin synthesis: Distribution of ribosomes on the messenger RNA for α and β chainsJournal of Molecular Biology, 1968
- Resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentationAnalytical Biochemistry, 1968
- The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte systemJournal of Molecular Biology, 1966
- Polyribosomes and control of protein synthesis: Effects of sodium fluoride and temperature in reticulocytesJournal of Molecular Biology, 1966
- Subribosomal particles and the transport of messenger RNA in HeLa cellsJournal of Molecular Biology, 1965
- A cytoplasmic particle bearing messenger ribonucleic acid in rat liverJournal of Molecular Biology, 1965
- Studies on the genesis of polyribosomes: I. Origin and significance of the subribosomal particlesJournal of Molecular Biology, 1965
- Initiation of hæmoglobin synthesis in cell-free systemsJournal of Molecular Biology, 1964
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics, 1959