Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.
- 1 May 1991
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (9) , 3734-3738
- https://doi.org/10.1073/pnas.88.9.3734
Abstract
CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.Keywords
This publication has 28 references indexed in Scilit:
- Microinjected centromere [corrected] kinetochore antibodies interfere with chromosome movement in meiotic and mitotic mouse oocytes [published erratum appears in J Cell Biol 1990 Dec;111(6 Pt 1):following 2800]The Journal of cell biology, 1990
- Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.The Journal of cell biology, 1990
- CENP-B: a major human centromere protein located beneath the kinetochore.The Journal of cell biology, 1990
- The motor for poleward chromosome movement in anaphase is in or near the kinetochore.The Journal of cell biology, 1989
- Polewards chromosome movement driven by microtubule depolymerization in vitroNature, 1988
- Molecular cloning of cDNA for CENP-B, the major human centromere autoantigenThe Journal of cell biology, 1987
- Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.The Journal of cell biology, 1987
- Fractionation and initial characterization of the kinetochore from mammalian metaphase chromosomes.The Journal of cell biology, 1985
- The kinetochore is part of the metaphase chromosome scaffold.The Journal of cell biology, 1984
- Kinetochore structure, duplication, and distribution in mammalian cells: analysis by human autoantibodies from scleroderma patients.The Journal of cell biology, 1981