Regulatory regions in the promoters of theSaccharomyces cerevisiae PYC1andPYC2genes encoding isoenzymes of pyruvate carboxylase

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Abstract
We have identified regions in the promoters of the PYC1 and PYC2 genes from Saccharomyces cerevisiae involved in their regulation in different culture conditions. In the case of PYC1, a UAS in the region between −330/−297 and three repressing sequences with the common central core CCGCC at positions −457, −432 and −399 were identified. Specific binding of nuclear proteins to the −330/−214 DNA fragment was abolished in rtg mutants suggesting a role for the RTG genes in the control of PYC1 expression. In the case of the PYC2 promoter, elimination of a fragment from −417 to −291 brings about a two-fold decrease in the expression in repressed conditions and a similar increase in derepression.