On the Oligomycin-Sensitivity and Subunit Composition of the ATPase Complex from Rhodospirillum rubrum
Open Access
- 1 April 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 34 (3-4) , 229-232
- https://doi.org/10.1515/znc-1979-3-412
Abstract
Two alternative procedures for isolation of the oligomycin-sensitive ATPase complex (E. C. 3.6.1.3) from R. rubrum chromatophores are compared with respect to ease, rapidity, and yield. The inhibitory effect of oligomycin on the membrane-bound Ca2+ -ATPase activity is increased during storage of the chromatophores, whereas the effect of oligomycin on the membrane-bound Mg2+ -ATPase activity does not change within a week. Oligomycin-sensitivity of the solubilized ATPase complex depends on the isolation procedure. The enzyme complex consists of at least nine different polypeptides with the apparent molecular weights of (1) 67,000, (2) 60,000, (3) 56,000, (4) 35,000, (5) 22,000, (6) 20,000, (7) 17,000, (8) 12,000, (9) 11,000. The polypeptides 2 - 4, 7, and 8 represent subunits of coupling factor 1.Keywords
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