Etude ultramicroscopique des modifications cytologiques chez le blé d'hiver lors de l'endurcissement au froid

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Abstract
This paper describes a few cytological and ultrastructural modifications during the hardening process of two varieties of winter wheat differing in frost resistance. In leaf cells of the hardier variety, Kharkov, subjected to hardening conditions, the quick and complete disappearance of starch grains in chloroplasts, the undulation of nuclear and chloroplast membranes, and an increase in osmiophilic granules have been observed. In the less-hardy variety, Selkirk, after a month of hardening, starch grains persist in chloroplasts, osmiophilic granules diminish in number and intensity, and vesicles are formed between the double membrane surrounding the chloroplasts. These vesicles are finally liberated in the ground cytoplasm. Their role could be the elimination of substances such as denatured proteins of the chloroplasts.

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