Studies on the association of the high mobility group non-histone chromatin proteins with isolated nucleosomes
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (1) , 167-179
- https://doi.org/10.1093/nar/6.1.167
Abstract
Nucleosomes have been isolated from rabbit thymus by sucrose gradient centrifugation, and their high mobility group (HMG) protein content analysed by electrophoresis on polyacrylamide gels. The results suggest that proteins HMG 14 and HMG 17 are associated with the core particle of the nucleosome, and that there are two or more sub-populations of both HMG 1 and HMG 2 molecules. One sub-population appears to be fairly tightly bound to the nucleosome, while another is rapidly released from the chromatin by digestion with micrococcal nuclease. The latter fraction may participate in a higher order folding of the nucleosomes.Keywords
This publication has 16 references indexed in Scilit:
- Chromatin structure through the cell cycle. Studies with regeneration rat liverBiochemical Journal, 1978
- A study of the localization of high mobility group proteins in chromatinCanadian Journal of Biochemistry, 1978
- Are the high mobility group non-histone chromosomal proteins associated with ‘active’ chromatin?Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatinCell, 1977
- Nucleosomes and subnucleosomes: heterogeneity and compositionCell, 1977
- Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.Proceedings of the National Academy of Sciences, 1977
- Structure of ChromatinAnnual Review of Biochemistry, 1977
- The interaction of nonhistone chromosomal proteins HMG1 and HMG2 with subfractions of H1 histone immobilized on agaroseBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- The presence of high mobility group non‐histone chromatin proteins in isolated nucleosomesFEBS Letters, 1977
- Chapter 15 The Isolation and Purification of the High Mobility Group (HMG) Nonhistone Chromosomal ProteinsPublished by Elsevier ,1977