Monoclonal antibody to the δ opioid receptor acts as an agonist in dual regulation of adenylate cyclase in NG108‐15 cells
- 28 July 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 456 (1) , 126-130
- https://doi.org/10.1016/s0014-5793(99)00878-9
Abstract
Monoclonal antibodies generated against multiple antigenic peptides of the N‐terminal sequence (3LVPSARAELQSSPLV17) of the cloned δ opioid receptor immunoprecipitated a 58 kDa protein from CHAPS‐solubilized NG108‐15 membranes. The immunoprecipitates bound [3H]DPDPE – but not [3H]DAMGO – with a K d of 6.4 nM and a B max of 75 pM. Western blot analysis revealed a distinct band of 58 kDa. The antibodies inhibited basal and PGE1‐stimulated cAMP levels, and mimicked the effect of agonists manifest in a compensatory increase in cAMP formation. The antibody will be potentially useful in the analysis of functional epitopes on the δ opioid receptor.Keywords
This publication has 20 references indexed in Scilit:
- Cloning and pharmacological characterization of a rat kappa opioid receptor.Proceedings of the National Academy of Sciences, 1993
- Cloning and pharmacological characterization of a rat μ opioid receptorNeuron, 1993
- Purification and reconstitution of the δ opioid receptorFEBS Letters, 1993
- Cloning of a Delta Opioid Receptor by Functional ExpressionScience, 1992
- The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.Proceedings of the National Academy of Sciences, 1992
- Method for isolation of kappa-opioid binding sites by dynorphin affinity chromatographyJournal of Neuroscience Research, 1990
- Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptorsNeuroscience Letters, 1987
- Purification of the opiate receptor of NG108-15 neuroblastoma-glioma hybrid cells.Proceedings of the National Academy of Sciences, 1985
- Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance.Proceedings of the National Academy of Sciences, 1975
- Morphine receptors as regulators of adenylate cyclase activity.Proceedings of the National Academy of Sciences, 1975