IMPROVED PLASMA CULTURE SYSTEM FOR THE PRODUCTION OF MEGAKARYOCYTE COLONIES INVITRO
- 1 January 1979
- journal article
- research article
- Vol. 7 (7) , 345-351
Abstract
A modified culture system was developed to grow and quantitate megakaryocyte colonies from mouse bone marrow more efficiently than previously described. Using this method, 30% of CFU-M [colony-forming unit, megakaryocyte] in normal marrow cells and 70 to 90% of CFU-M in regenerating marrow cells were killed by high specific activity 3H-TdR [thymidine] in vitro. CFU-M are apparently proliferating even in normal adult hemopoietic tissue, but the proportion of cells that are proliferating is greater in regenerating marrow than in normal intact mice.This publication has 3 references indexed in Scilit:
- REGULATION OF PROLIFERATION OF MURINE MEGAKARYOCYTE PROGENITOR CELLS BY CELL-CYCLE1978
- REGULATION OF MEGAKARYOPOIESIS IN LONG-TERM MURINE BONE-MARROW CULTURES1978
- THE ROLE OF ERYTHROPOIETIN IN REGULATION OF POPULATION SIZE AND CELL CYCLING OF EARLY AND LATE ERYTHROID PRECURSORS IN MOUSE BONE MARROWCell Proliferation, 1977