Regulation by phosphorylation of the zinc finger protein KRC that binds the B motif and V(D)J recombination signal sequences

Open Access

1 January 1999

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 27 (2) , 643-648
https://doi.org/10.1093/nar/27.2.643

Abstract

The DNA binding protein KRC (for κB binding and recognition component of the V(D)J recombination signal sequence) belongs to a family of large zinc finger proteins that bind to the κB motif and contains two widely separated DNA binding structures. In addition to the κB motif, KRC fusion proteins bind to the signal sequences of V(D)J recombination to form highly ordered complexes. Here, we report that KRC may be regulated by post-translational modifications. Specific protein kinases present in the nucleus of pre-B cells phosphorylated a KRC fusion protein at tyrosine and serine residues. Such protein modifications increased DNA binding, thereby providing a mechanism by which KRC responds to signal transduction pathways. KRC is a substrate of epidermal growth factor receptor kinase and P³⁴cdc2 kinase in vitro. Our results suggest that activation of the KRC family of transcription factors may provide a mechanism by which oncogenic tyrosine kinases regulate genes with κB-controlled gene regulatory elements.

Keywords

This publication has 26 references indexed in Scilit:

The Caenorhabditis elegans gene sem-4 controls neuronal and mesodermal cell development and encodes a zinc finger protein.
Genes & Development, 1996
The Mouse DNA Binding Protein Rc for the Kappa B Motif of Transcription and for the V(D)J Recombination Signal Sequences Contains Composite DNA–Protein Interaction Domains and Belongs to a New Family of Large Transcriptional Proteins
Genomics, 1996
schnurri is required for drosophila Dpp signaling and encodes a zinc finger protein similar to the mammalian transcription factor PRDII-BF1
Cell, 1995
The drosophila schnurri gene acts in the Dpp/TGFβ signaling pathway and encodes a transcription factor homologous to the human MBP family
Cell, 1995
The V(D)J recombination signal sequence and χB binding protein Rc binds DNA as dimers and forms multimeric structures with its DNA ligands
Nucleic Acids Research, 1994
Structure and expression of major histocompatibility complex-binding protein 2, a 275-kDa zinc finger protein that binds to an enhancer of major histocompatibility complex class I genes.
Proceedings of the National Academy of Sciences, 1992
Two genes encode factors with NF-kappa B- and H2TF1-like DNA-binding properties.
Proceedings of the National Academy of Sciences, 1990
A large protein containing zinc finger domains binds to related sequence elements in the enhancers of the class I major histocompatibility complex and kappa immunoglobulin genes.
Molecular and Cellular Biology, 1990
A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence.
Genes & Development, 1990
Molecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNA
Cell, 1988