The Role of Macrophages in the Chemotactic Response of Polymorphonuclear Leukocytes to Bacterial Lipopolysaccharides

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Abstract
C3H/HeJ mice do not respond to the toxic and immunological properties of LPS [lipopolysaccharide] whereas histocompatible C3HeB/FeJ do respond. The unresponsiveness of C3H/HeJ strain is genetically determined and occurs primarily at the cellular level. The peritoneal PMN [polymorphonuclear leukocytes] influx induced after i.v. LPS administration in C3H/HeJ recipients that received i.p. different C3HeB/FeJ or C3H/HeJ peritoneal cells was studied. A significant PMN migration into the peritoneal cavity occurs after i.v. LPS administration only in C3H/HeJ recipients previously inoculated with C3HeB/FeJ unseparated or adherent peritoneal cells. The peritoneal PMN influx after i.v. LPS injection was not observed in C3H/HeJ recipients that received i.p. C3HeB/FeJ nonadherent peritoneal cells or C3H/HeJ unseparated peritoneal cells. The peritoneal chemotactic signal is triggered after the interaction of LPS with the adherent peritoneal cell population.