COMPARISON OF 2 DIRECT ASSAYS FOR PLATELET-ASSOCIATED IGG (PAIGG) IN ASSESSMENT OF IMMUNE AND NON-IMMUNE THROMBOCYTOPENIA

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 55  (3) , 424-429
Abstract
A number of methods were developed to measure platelet-associated Ig[immunoglobulin]G (PAIgG). The antiglobulin consumption assay directly quantitates IgG on the platelet and is sensitive and specific. A fluorescent anti-IgG assay was recently described and has the advantage of simplicity. The results of these 2 PAIgG assays were compared in immune and nonimmune thrombocytopenia and nonthrombocytopenic controls. The antiglobulin consumption assay was negative in 61 of 62 and the fluorescent negative in 54 of 62 assays in nonthrombocytopenic controls, and they were negative in 11 of 13 and 8 of 13 assays, respectively, in nonimmune thrombocytopenic patients. The antiglobulin consumption assay was positive in 54 of 58 and the fluorescent positive in 24 of 58 assays of patients with immune thrombocytopenia (ITP [idiopathic thrombocytopenic purpura] and SLE [systemic lupus erythematosus]). The overall sensitivity and specificity of the antiglobulin consumption assay was 94% and 95% and of the fluorescent assay was 44% and 82%.