Sodium Pentobarbital Effects on Cardiac Function and Response to Dobutamine

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Abstract
Summary: This study was undertaken to determine if sodium pentobarbital had persistent effects on isolated working rat heart function that altered the response of the heart to subsequently administered dobutamine. The effects of in vitro sodium pentobarbital treatment were studied using isolated working hearts from rats that were killed after pentobarbital anesthesia or by cervical fracture. Hemodynamic and contractile functions of the isolated hearts were significantly depressed in the presence of 0.4 mM sodium pentobarbital in vitro. After the pentobarbital was washed out of the preparation, all indices of cardiac function recovered fully except for −dP/dtmax, which remained depressed by ˜7%. The subsequent inotropic response of the hearts to 5 × 10−8M dobutamine was minimally affected by the prior exposure to pentobarbital in vitro. The data confirmed that hearts from rats killed by cervical fracture exhibited a smaller inotropic response to dobutamine than hearts from pentobarbital-anesthetized rats. In addition, hearts excised from ventilated animals had significantly higher left ventricular peak systolic pressure, peak aortic flow rate, and peak power than hearts from nonventilated animals, indicating the importance of ventilating the animal during cardiac excision in order to obtain optimum isolated heart function.