Differential Hybridization with Cloned cDNA Sequences for Detecting a Specific Isolate of Citrus Tristeza Virus
- 1 January 1986
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 76 (8) , 820-824
- https://doi.org/10.1094/phyto-76-820
Abstract
Two plasmids with cDNA inserts representing different regions of the citrus tristeza virus (CTV) genome were used to compare five biologically distinct isolates of CTV. One of the plasmids (D2) hybridized strongly with all of the isolates. The other plasmid (D1) hybridized strongly with four of the isolates. A fifth isolate, which caused severe symptoms, hybridized weakly, if at all, with D1. Similarresults were obtained using total plant RNA, RNA extracted from purified and partially purified virus preparations, or dsRNA preparations. Virion RNA of two CTV isolates, HT and VT, used as probes for hybridization with restriction patterns of the D1 CTV clone revealed DNA fragments that carry strain specific sequences.This publication has 4 references indexed in Scilit:
- Molecular Cloning of Complementary DNA Sequences of Citrus Tristeza Virus RNAJournal of General Virology, 1983
- A simple procedure for the extraction of double-stranded RNA from virus-infected plantsJournal of Virological Methods, 1983
- Double-Stranded RNA from Plants Infected with ClosterovirusesPhytopathology®, 1983
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976