Function of a yeast TATA element-binding protein in a mammalian transcription system

Abstract
Saccharomyces cerevisiae contains a protein which is functionally similar to the mammalian TATA element-binding transcription factor, TFIID. The yeast factor substitutes for TFIID in a mammalian RNA polymerase II in vitro transcription system, forms a stable preinitiation complex on the Adenovirus-2 major late promoter, and binds specifically to the TATA boxes of the viral promoter and the yeast CYCl promoter. Interestingly, the yeast factor promotes initiation at a distance from the TATA element typical of a mammalian system.