Characteristics and Functional Specificity of Anti-Human BAT (Brain Associated Thymocyte Antigen) Serum

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Abstract
A rabbit antiserum to human fetal brain after multiple absorption reacted with 100% of thymocytes, 55% of peripheral blood lymphocytes and 90% of enriched T [thymus-derived] lymphocytes, but not significantly with B [bone marrow-derived] lymphocytes. Spontaneous SRBC [sheep red blood cell] rosette formation was inhibited by anti-BAT [brain associated thymocyte antigen] pretreatment; EAC-rosette formation remained unaffected. The antiserum itself was highly stimulatory. Cells treated with the antiserum and complement exhibited marked inhibition of responsiveness to Con [concanavalin] A, little effect with PHA [phytohemagglutinin] and no alteration with PWM [pokeweed mitogen]. The MLC [mixed lymphocyte cultured] reaction was inhibited only when the responder cells were treated with the antiserum and complement caused a dose-dependent suppression of blastogenic response to PPD [purified protein derivative] and n[native]DNA. No effect was noted in MIF [migration inhibitory factors] producing cells. Con A-induced suppressor function of lymphocytes was abolished by treatment with the antiserum and complement. The anti-BAT serum obtained apparently can be utilized for the isolation of T lymphocyte subsets.