Rapid Chemical Changes in Reconstituted Dry Milk

Abstract
Two different chemical analyses were used with 6 samples of reconstituted non-fat dry milk. Samples were analyzed for 5-hydroxymethylfurfural (HMF) and for indophenol-reducing substances. HMF did not measure free 5-hydroxymethylfurfural in the milks but rather the amount formed under standardized digestion conditions. Indophenol titrations are expressed as molar concentration of diolreductone. Samples were prepared by shaking 10 g milk powder with 100 ml distilled H2O. Aliquot portions of the reconstituted samples (stored at 24[degree]C) were removed at specified times for analyses. The 1st samples were removed 3 minutes after the start of reconstitution. Results indicate that rapid chemical changes occurred in freshly reconstituted instant milks. Changes in non-instant milks appeared to be less pronounced and more variable than in the former.
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