Clonal history of a human follicular lymphoma as revealed in the immunoglobulin variable region genes
- 1 March 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 86 (3) , 505-512
- https://doi.org/10.1111/j.1365-2141.1994.tb04780.x
Abstract
Summary. The variable region genes used to encode the immunoglobulin expressed by tumour cells of a patient with follicular lymphoma have been identified and sequenced. Initially, a lymph node biopsy was analysed and revealed usage of VH and Vκ genes which had numerous substitutions as compared with the closest germ line genes. The pattern of mutations in VH was consistent with a role for positive selection by antigen. In addition, there was evidence in both VH and Vκ sequences for clonal heterogeneity. After 5 years, which included treatment with chemotherapy, the patient relapsed with tumour cells present in the blood. Analysis of the V‐genes used by the emerging tumour revealed a single homogeneous sequence for both VH and VL, which, in each case, matched closely one of the sequences in the original lymph node biopsy. These results indicate that selection. possibly mediated by antigen, can operate on a cell destined to give rise to lymphoma, and that intraclonal variation can occur after the neoplastic event. However, in this case, late relapse in the blood is dominated by a single clone.Keywords
This publication has 24 references indexed in Scilit:
- A Genetic Approach to Idiotypic VaccinationJournal of Immunotherapy, 1993
- Immunological MemoryAnnual Review of Immunology, 1993
- The repertoire of human germline vH sequences reveals about fifty groups of VH segments with different hypervariable loopsJournal of Molecular Biology, 1992
- Clonal expansion in follicular lymphoma occurs subsequent to antigenic selection.The Journal of Experimental Medicine, 1992
- Intraclonal generation of antibody mutants in germinal centresNature, 1991
- The role of clonal selection in the pathogenesis of an autoreactive human B cell lymphoma.The Journal of Experimental Medicine, 1991
- Mechanism of antigen-driven selection in germinal centresNature, 1989
- Multiple Recurrent Genomic Defects in Follicular LymphomaNew England Journal of Medicine, 1987
- Antigen‐Driven Selection of Virgin and Memory B CellsImmunological Reviews, 1986
- Human immunoglobulin D segments encoded in tandem multigenic familiesNature, 1981