Persistent colonization of carbon dioxide incubators withCandida parapsilosis

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Abstract
Recurrent contamination of bacteriological specimens withCandida parapsilosis led to epidemiological investigations which indicated persistent colonization of carbon dioxide incubators as the most likely source. Changes in the technical arrangements and institution of a meticulous cleansing protocol eliminated contamination of specimens but not colonization of the incubators. Tests for tolerance of 17 % NaCl and survival at 50 °C, and SDS-PAGE analysis of crude cell extracts allowed discrimination between epidemic and non-epidemic isolates, while enzyme profile analysis and susceptibility studies failed as typing methods.