Characterization of soluble uterine cyclic nucleotide phosphodiesterase

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Abstract
Soluble cyclic nucleotide phosphodiesterase [EC 3.1.4.17] of rat uterus displays distinct structural and regulatory properties. Like phosphodiesterases from many mammalian sources the soluble uterine enzyme system exhibits nonlinear Lineweaver-Burk kinetics with c[cyclic]AMP as substrate (apparent Km .simeq. 3 and 20 .mu.M) and linear kinetics with cGMP as substrate (apparent Km .simeq. 3 .mu.M). Unlike most other mammalian phosphodiesterases, numerous separation procedures reveal only a single form of uterine phosphodiesterase which catalyzes the hydrolysis of both cAMP and cGMP. A single form of the enzyme is observed upon sucrose gradient centrifugation (7.9 S), agarose gel filtration and DEAE-cellulose chromatography at either pH 8.0 or 6.0. Heat denaturation (50.degree. C) of soluble uterine phosphodiesterase causes the loss of both cAMP and cGMP hydrolytic activities at the same rate. Isoelectric focusing reveals major (pI = 5.2) and minor forms (pI = 5.8) of phosphodiesterase which both catalyze the hydrolysis of the 2 cyclic nucleotide substrates. In vivo administration of estradiol produces identical decreases in the activities of cAMP and cGMP phosphodiesterase. The uterus may contain a single form of soluble phosphodiesterase which catalyzes the hydrolysis of both cAMP and cGMP.