Detection of Pseudocercosporella herpotrichoides (Fron) Deighton in Wheat by Indirect ELISA

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Abstract
An indirect ELISA for quantitative detection of P. herpotrichoides in fections in wheat is presented. All tested isolates of the virulent varieties P. herpotrichoides var. herpotrichoides, P. h. var. acuformis or the W‐ and R‐type react on a high level in the test, while the less virulent P. anguioides is assessed only with 40% and the avirulent P. aestiva with 20% of the homologous reaction. No crossreactions occur with extracts of 11 other species of in, vitro cultivated fungi nor with plant material infected with other pathogens. The infection profile throughout the leaf‐sheaths was clearly reflected by ELISA. The examination of 24 stembase samples from the field showed that the values assessed by ELISA correlate well also with the disease indices of naturally infected plant material.