Sexual co-flocculation and asexual self-flocculation in budding and fission yeasts: experimental establishment of a fundamental difference

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Abstract
Heavily flocculated yeasts were resuspended in a variety of sugar syrups and solutions. Asexual Saccharomyces floes remained patent in water and in 50% galactose but dispersed in 50% mannose and in 10 mM EDTA. Asexual Schizosaccharomyces floes (either h− or h+) remained patent in water and in 50% mannose but dispersed in 50% galactose and 10 mM EDTA. Sexual Schizosaccharomyces floes (homothallic NCYC 132, or heterothallic h− and h+ combined) remained patent in water, in 50% galactose, in 50% mannose, and in 10 mM EDTA. Dispersal of asexual floes in sugar syrups and EDTA suggests that asexual flocculation is mediated by protein–carbohydrate lectinlike interactions stabilized by Ca2+. Failure of the sexually flocculent fission yeasts to disperse in any of these diagnostic solutions suggests that sexual flocculation is mediated by protein–protein interactions in which Ca2+ is irrelevant.

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