Horn Flies: Emergence, Survival, and Sterility After 60Co Irradiation of Pupae13

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Abstract
Irradiation of colony-reared 30-h-old pupae of Haematobia Irritans (L.) with 2500 rad from 60CO completely inhibited emergence of flies; with irradiation of 54-h-old pupae, some flies emerged, but all died within 3 days. When 78-h-old pupae were irradiated, unusual numbers of adults died within 3 days. However, when pupae were 102 or 126 h old at the time of irradiation, neither emergence nor survival of flies was affected adversely. Also, when this dose was applied after emergence had begun (avg pupal age 120 h), both males and females were sterile. With a dose of 2000 rad, the females were sterile, and fewer than 1.0% of the males were fertile. Male flies eclosing during the initial 24 h of the emergence period accounted for most of this fertility.

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