Plasma Determination of Flumazenil, a Benzodiazepine Antagonist, by Immunotoxicology and by Capillary Gas Chromatography/Mass Spectrometry
- 1 July 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 15 (4) , 202-203
- https://doi.org/10.1093/jat/15.4.202
Abstract
The method presented describes a sensitive and specific quantitative assay for the determination of flumazenil in human plasma. Flumazenil and an internal standard, midazolam, are isolated by a basic extraction. The final extract is separated on a 25-m BP-1 capillary column and drugs are detected by selected ion monitoring at m/z 229 and m/z 310 for flumazenil and the internal standard, respectively. The minimum detectable quantity is 1.0 ng/mL, for flumazenil in plasma. Coefficients of variation for within-run data were less than 6%.Keywords
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