Simultaneous competitive enzyme immunoassay for human chorionic gonadotropin.
- 1 January 1978
- journal article
- research article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 25 (5) , 515-517
- https://doi.org/10.1507/endocrj1954.25.515
Abstract
A simultaneous competitive enzyme immunoassay (SICEIA) for hCG [human chorionic gonadotropin] was developed using .beta.-D-galactosidase (.beta.-Gal) as a labeled enzyme and anti-hCG antibody coated sheep red blood cells (SRBC) as a solid phase. In this report, a new coupling agent, MCAE [N-hydroxy-succinimide ester of 4-(maleimidomethyl) cyclohexane-carboxylic acid], was used to couple .beta.-Gal with hCG. The sensitivity was improved to the degree of 2.5 mIU/ml, equal to that of RIA [radioimmunoassay]. The present procedure was safer and more rapid than RIA. The value of hCG in urine by the new procedure had good correlation with that by RIA.This publication has 1 reference indexed in Scilit:
- Enzyme Coupled Immunoassay of Insulin Using a Novel Coupling ReagentThe Journal of Biochemistry, 1976