RED-CELL LYSIS COUPLED TO PEROXIDATION OF LIVER MICROSOMAL LIPIDS - COMPARTMENTALIZATION OF HEMOLYTIC SYSTEM

  • 1 January 1977
    • journal article
    • research article
    • Vol. 17  (3) , 519-528
Abstract
As a contribution to understanding the mechanisms by which addition of erythrocytes [rat] to liver microsomes incubated in the NADPH dependent system results in hemolysis, experiments are presented in which the system containing actively peroxidizing microsomes was separated from the system containing the red blood cells by a dialysis membrane. When liver microsomes were incubated with NADPH, lipid peroxidation, as measured by the formation of malonic dialdehyde (MDA), rapidly took place. The MDA concentration tended to equilibrate across the dialysis membrane. After a lag phase of 1 h, lysis of erythrocytes contained in the dialysis tube started. It reached its maximal level (> 80%) at 110 min of incubation. Toxic products originating during the course of lipid peroxidation may induce pathological effect at distant loci. The accomplishment of a compartmentalization of the 2 systems involved in the reaction may offer an approach to the recognition of the toxic factors.