Intermediates in 16‐androstene biosynthesis in neonatal porcine testis
Open Access
- 13 February 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 167 (1) , 103-108
- https://doi.org/10.1016/0014-5793(84)80841-8
Abstract
The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3‐week old piglets. Metabolites identified after derivatization by capillary gas‐chromatography and further by combined gas chromatography‐mass spectrometry were: 17‐hydroxy‐ and 16α‐hydroxypregnenolone, dehydroepiandrosterone, 5‐androstenediols, 5‐pregnenediol and andien‐β. Pregnenolone and 16‐dehydropregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas‐chromatography.Keywords
This publication has 19 references indexed in Scilit:
- The validation and use of a radioimmunoassay for 5α-androst-16-en-3-one in human axillary collectionsPublished by Elsevier ,2003
- Estimation of the odorous steroid, 5α-androst-16-en-3-one, in human salivCellular and Molecular Life Sciences, 1983
- The influence of boar-related odours on puberty attainment in giltsAnimal Science, 1983
- Pig courtship behaviour: pheromonal property of androstene steroids in male submaxillary secretionAnimal Science, 1980
- Formation of [17-2H]androsta-5,16-dien-3β-ol from [17,21,21,21]pregnenolone by the microsomal fraction of boar testisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- Simultaneous estimation of urinary steroids by semiautomated gas chromatography. investigation of neo-natal infants and children with abnormal steroid synthesisClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- THE IDENTIFICATION OF C19-16-UNSATURATED STEROIDS AND ESTIMATION OF 17-OXOSTEROIDS IN BOAR SPERMATIC VEIN PLASMA AND URINEJournal of Endocrinology, 1970
- Gas chromatographic and mass spectrometric studies of oximes derived from 20-oxosteroidsSteroids, 1970
- Steroids in Germfree and Conventional RatsEuropean Journal of Biochemistry, 1968
- Identifiction of 3β, 17β-dihydroxyandrost-5-ene, 3β,20α-dihydroxypregn-5-ene and epiandrosterone in human peripheral bloodSteroids, 1966