PHENOTYPE OF RAT NATURAL-KILLER CELLS DEFINED BY MONOCLONAL-ANTIBODIES MARKING RAT LYMPHOCYTE SUBSETS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 45  (1) , 97-103
Abstract
The fluorescence-activated cell sorter and a rosette-depletion technique were used to separate rat spleen lymphocytes and BCG-activated lymphocytes into subpopulations with and without the antigens defined by W3/13, W3/15 and OX8 monoclonal antibodies. The resultant populations were then tested for natural killer (NK) activity in a quantifiable 6-h 51Cr release assay. Rat natural killer cells appear to be heterogeneous with respect to their surface antigen expression and subpopulations of rat NK cells express the OX8 and W3/13 defined antigens. Rat NK cells do not express the antigen defined by W3/25 monoclonal antibody.