Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatography
- 1 December 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (18) , 4678-4683
- https://doi.org/10.1002/pmic.200500002
Abstract
Phosphoproteome studies are hampered by the lack of methods which allow a comprehensive and fast analysis of intact phosphoproteins. Here we describe an immobilized metal‐affinity chromatography (IMAC)‐based technique for the enrichment of phosphorylated proteins, which allows recovery of up to 90% of phosphoproteins. This technique is compatible with 2‐DE and can be applied to cultured cells and tissues.Keywords
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