Protection of Neocortical Tissue Prisms from Freeze‐Thaw Injury by Dimethyl Sulphoxide
- 1 July 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 37 (1) , 243-246
- https://doi.org/10.1111/j.1471-4159.1981.tb05315.x
Abstract
Neocortical tissue prisms prepared from rat and human brain were frozen to -196°C by a two-step freezing procedure and 10% dimethyl sulphoxide as cryoprotectant. Frozen and thawed rat neocortical prisms incorporated glucose into acetylcholine and carbon dioxide at 89% and 86% of control values, respectively, and noradrenaline uptake into frozen and thawed rat prisms was 94% of the control value. Frozen and thawed prisms from three human neocortical specimens showed a similar degree of protection from freeze-thaw injury.Keywords
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