Identification by flow cytometry of two distinct rhodamine‐123‐stained mitochondrial populations in rat liver
- 28 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 254 (1-2) , 115-120
- https://doi.org/10.1016/0014-5793(89)81020-8
Abstract
Isolated rat liver mitochondria were split into three fractions of increasing density when applied to a Percoll gradient. NADH‐ubiquinone oxidoreductase, succinate dehydrogenase and cytochrome‐c oxidase but not F1‐ATPase activities increased with density as well as respiratory rate in state 3 and the respiratory control index. Flow cytometry of mitochondrial density fractions stained with rhodamine‐123 revealed the occurrence in each density fraction of two distinct mitochondrial populations with different fluorescence intensity. The high fluorescence population was minor and its proportion decreased with density. The extent of high fluorescence population staining depended on the deenergized state of the mitochondria suggesting that this population represents an immature form of the mitochondria which may develop into a fully functional organelle by the incorporation of structural and/or functional proteins.Keywords
This publication has 22 references indexed in Scilit:
- Use of Rhodamine 123 to investigate alterations in mitochondrial activity in isolated mouse liver mitochondriaBiochemical and Biophysical Research Communications, 1988
- Rhodamine 123 inhibits import of rat liver mitochondrial transhydrogenaseBiochemical and Biophysical Research Communications, 1987
- Binding of concanavalin A to the outer membrane of potato tuber mitochondria detected by flow cytometryFEBS Letters, 1986
- Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometryBiology of the Cell, 1986
- Rhodamine 123 inhibits bioenergetic function in isolated rat liver mitochondriaBiochemical and Biophysical Research Communications, 1984
- A one-step determination of serum 5'-nucleotidase using a centrifugal analyzerClinica Chimica Acta; International Journal of Clinical Chemistry, 1982
- Status of mitochondria in living human fibroblasts during growth and senescence in vitro: use of the laser dye rhodamine 123.The Journal of cell biology, 1981
- Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.The Journal of cell biology, 1981
- The viability of cells grown or centrifuged in a new density gradient medium, Percoll(TM)Experimental Cell Research, 1977
- Mitochondrial heterogeneity in foetal and suckling rat liver: Differential leucine incorporation into the proteins of two mitochondrial populationsBiochemical and Biophysical Research Communications, 1976