High Speed Separations of Nucleotides and Nucleic Acid Bases by Column Chromatography Using Controlled Surface Porosity Ion Exchangers

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Abstract
Rapid separations of nucleotides and nucleic acid bases can be carried out by column chromatography using new “Zipax” controlled surface porosity anion and cation exchange packings. For example, the four main ribonucleoside-5'-mono-phosphoric acids may be completely separated in about six minutes using a strongly basic anion exchange column containing nonporous, spherical support particles uniformly coated with a thin, porous layer of sorbent. The high speed capabilities of the new ion exchangers are primarily due to the greatly improved mass transport effects as compared with conventional ion exchange resins. For analytical studies, the exchangers are normally used in 2 or 3 mm, i.d., columns; consequently, small samples are employed in conjunction with a sensitive, continuous detector. The hard, spherical nature of the new exchangers permit their operation at high column input pressures. Carrier velocities in excess of 3 cm/sec may be employed with good results.

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