Incorporation of Glycerol-3-Phosphate into the Hepatic Lipids of Rainbow Trout, Salmo gairdneri

Abstract
The incorporation of sn-[14C]glycerol-3-phosphate into the liver lipids of rainbow trout (Salmo gairdneri) was studied using a homogenate preparation. The reaction was dependent upon added palmitoyl-CoA as a source of activated fatty acid and lysophosphatidic acid as well as phosphatidic acid were found by means of thin-layer chromatography to be important metabolic products. The optimal conditions for studying glycerol-3-phosphate acyltransferase activity in the liver of rainbow trout are given. The maximum rate of entry of glycerol-3-phosphate into lipid esters was 91 μ moles/g liver per hour.

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