The biochemistry of aromatic amines. 3. Enzymic hydroxylation by rat-liver microsomes

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Abstract
The enzyme system responsible for the conversion of 2-acetamidonaphthalene into 2-acetamido-6-naphthol is associated with the microsomes of rat liver and requires reduced triphosphopyridine nucleotide (TPNH) and oxygen. The TPNH can be replaced by triphosphopyridine nucleotide, glucose 6-phosphate (G 6-P) and G 6-P dehydrogenase, and conditions affecting the rate of hydroxylation of 2-acetamidonaphthalene by this system have been studied. The system is not specific for 2-acetamidonaphthalene but will hydroxylate other aromatic amines, N-substituted amines and also benzene and naphthalene.