An Unexpected Major Groove Binding of Netropsin and Distamycin A to tRNAphe
- 31 July 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 2 (1) , 165-174
- https://doi.org/10.1080/07391102.1984.10507555
Abstract
Crystalline complexes of yeast tRNAphe and the oligopeptide antibiotics netropsin and distamycin A were prepared by diffusing drugs into crystals of tRNA. X-ray structure analyses of these complexes reveal a single common binding site for both drugs which is located in the major or deep groove of the tRNA T-stem. The netropsin-tRNA complex is stabilized by specific hydrogen bonds between the amide groups of the drug and the tRNA bases G51 0(6), U52 0(4) and G53 N(7) on one strand, and is further stabilized by electrostatic interactions between the positively charges guanidino side chain of the drug and the tRNA phosphate P53 on the same strand and the positively charged amidino propyl side chain and the phosphates P61, P62 and P63 on the opposite strand of the double helix. These results are in contrast to the implicated minor groove binding of these drugs to non-guanine sequences in DNA. The binding to the GUG sequence in tRNA implies that major groove binding to certain DNA sequences is possible.This publication has 16 references indexed in Scilit:
- X-ray diffraction study of the zinc(II) binding sites in yeast phenylalanine transfer RNA preferential binding of zinc to guanines in purine-purine sequencesBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Netropsin, a DNA-binding oligopeptide structural and binding studiesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- Crystal and molecular structure of yeast phenylalanyl transfer RNA. Structure determination, difference Fourier refinement, molecular conformation, metal and solvent bindingActa Crystallographica Section B: Structural Science, Crystal Engineering and Materials, 1978
- A crystallographic study of metal-binding to yeast phenylalanine transfer RNAJournal of Molecular Biology, 1977
- Idealized atomic coordinates of yeast phenylalanine transfer RNABiochemical and Biophysical Research Communications, 1976
- Three-Dimensional Structure of Yeast Phenylalanine Transfer RNA: Folding of the Polynucleotide ChainScience, 1973
- Interaction of the oligopeptide antibiotics netropsin and distamycin a with nucleic acidsJournal of Molecular Biology, 1971
- Structure and Synthesis of Distamycin ANature, 1964
- THE STRUCTURE OF ANTIBIOTIC T-1384Journal of the American Chemical Society, 1957
- Netropsin, a New Antibiotic Produced by a StreptomycesJournal of the American Chemical Society, 1951