THE EARLY PHASE OF THYROID HORMONE FORMATION: STUDIES IN TISSUE SLICES1

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Abstract
Sheep thyroid slices were incubated for various periods of time in buffered solutions containing I131. The nature and the distribution of labeled components were determined. The slices accumulated up to 70% of flask I m during 1 hour, but a large fraction of this iodide was not bound to protein as PBI131. Slices were homogenized and PBI131 content of the separated nuclear, mitochondrial, microsomal, and supernatant fractions of the homogenate was determined. No definite evidence of transfer of particulate PBI131 to the soluble fraction of the coll was obtained, although this phenomenon was suggested by some experiments. Labeled MIT and DIT were present in thyroglobulin within 2 minutes after start of incubation, but thyroxine and triiodothyronine were not identified. In most experiments DIT/MIT ratio increased gradually during incubation. The only soluble labeled protein identified was thyroglobulin.

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