Nucleotide sequence of phenylalanine transfer RNA from Schizosaccharomyces pombe: implications for transfer RNA recognition by yeast phenylalanyl-tRNA synthetase

1 May 1978

journal article
research article
Published by American Chemical Society (ACS) in Biochemistry

Vol. 17 (9) , 1622-1628
https://doi.org/10.1021/bi00602a007

Abstract

The nucleotide sequence of S. pombe tRNAPhe was determined to be pG-U-C-G-C-A-A-U-G**-G*-U-G-.psi.-A-G-D-D-G-G-G-A-G-C-A-.psi.-G*-A-C-A-G-A-Cm-U-Gm-A-A-Y-A-.psi.-m5C-U-G-U-U-G-m7G-U*-C-A-U-C-G-G-T-.psi.-C-G-A-U-C-C-C-G-G-U-U-U-G-U-G-A-C-A-C-C-AOH. This sequence differs from that of Saccharomyces cerevisiae tRNAPhe in 27 nucleotides. S. cerevisiae phenylalanyl-tRNA synthetase aminoacylates both the homologous tRNAPhe and S. pombe tRNAPhe; the reactions have similar Km and Vmax values. However, the nucleotide sequence in the D stem is different in the 2 tRNA. This region was proposed by Roe, B., et al. to be the major recognition site for yeast phenylalanyl-tRNA synthetase, but the present results cast doubt on the validity of this hypothesis.

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