CALIBRATION OF GEL PERMEATION CHROMATOGRAPHY MATERIALS FOR USE WITH HUMIC ACID

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Abstract
Summary: Three common gel‐chromatography materials have been calibrated for humic acid, using fractionated samples the molecular weights of which were calculated by combining sedimentation coefficients determined on the ultracentrifuge with diffusion coefficients.The gel materials were found to have the following fractionation ranges for humic acid when tris buffer was used: Sephadex G‐100, 2500 to 100000; Bio Gel P‐150, below 1000 to 40000; Sepharose 6B, 1500 to 2500000. Borax buffer appeared to extend the range of Sephadex G‐100 to lower molecular weights.A brief comparison is made of the retention behaviours of humic acid, proteins, and dextrans on the gel materials.