Salmonella Detection in Meat and Fish by Membrane Hybridization with Chromogenic/Phosphatase/Biotin DNA Probe
- 1 November 1991
- journal article
- Published by Wiley in Journal of Food Science
- Vol. 56 (6) , 1519-1523
- https://doi.org/10.1111/j.1365-2621.1991.tb08630.x
Abstract
The hybridization specificity of a biotin labeled 1.8 kb HindIII DNA fragment was confirmed by colony hybridization with Salmonella and non‐Salmonella isolates. Culture conditions were then tested for the enrichment of salmonellae in foods with large populations of competitive flora. Different conditions of preenrichment and selective enrichment could be used for detecting low populations of salmonellae in foods. Enrichment using lactose combined tetrathionate (CTET) broth and Salmonella‐Shigella (SS) agar followed by spotting the suspected isolates on a nitrocellulose membrane [CETE → SS (S)] was better. As low as 1.6 × 100 salmonellae/g food could be detected.Keywords
This publication has 15 references indexed in Scilit:
- Possible use of a 1.8 kb DNA fragment for the specific detection of Salmonella in foodsJournal of Fermentation and Bioengineering, 1989
- Comparison of Cultural Methods and the DNA Hybridization Test for Detection of Salmonellae in Ground BeefJournal of Food Science, 1987
- Comparative Study of a DNA Hybridization Method and the Conventional Culture Procedure for Detection of Salmonella in FoodsJournal of Food Science, 1987
- Evaluation of Direct Enrichment at Elevated Temperature for Recovery of Salmonellae from OystersJournal of Food Protection, 1984
- Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.Proceedings of the National Academy of Sciences, 1982
- DNA HYBRIDIZATION METHOD FOR DETECTING ENTEROTOXIGENIC ESCHERICHIA COLI IN HUMAN ISOLATES AND ITS POSSIBLE APPLICATION TO FOOD SAMPLES1Journal of Food Safety, 1981
- Recovery of Salmonellae from Foods using a Combined Enrichment TechniqueJournal of Food Science, 1981
- Detection of Enterotoxigenic Escherichia coli by DNA Colony HybridizationThe Journal of Infectious Diseases, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977