Loss of Subunit Association Ability of Escherichia coli Ribosomes by Washing and Its Recovery

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Abstract
The ribosomal subunits of Escherichia coli washed well with buffer containing 0.1 mM magnesium ion fail to associate with the partner subunits even at high (10 mM or more) magnesium ion concentrations when examined at low temperature. However, they recovered the ability to associate when they were preincubated at 37°C with magnesium and univalent (ammonium) ions. Preincubation of the 30S subunit only was sufficient for the recovery but that of the 50S subunit only was ineffective. Spermine, poly A, poly U or poly U and tRNA accelerated the recovery. Also, a factor contained in the post-ribosomal fraction was effective. From the study of magnesium dependence of poly U-directed polyphenylalanine synthesis, the 70S ribo-somes recovered with poly A or the supernatant factor were found to be different from those recovered with spermine or with uni- and divalent cations.

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