Detectability and Precision of the AOAC Bacillus stearothermophilus Disc Assay Demonstrated in the 1981 FDA Split Milk Sample Testing Program
- 1 February 1983
- journal article
- research article
- Published by Elsevier in Journal of Food Protection
- Vol. 46 (2) , 84-86
- https://doi.org/10.4315/0362-028x-46.2.84
Abstract
Four blind duplicate milk samples containing β-lactam residues were analyzed by 149 analysts at 47 laboratories. Four official variations were used: (a) antibiotic 4 (A4) agar and 55°C incubation, (b) A4 agar and 64°C incubation, (c) PM indicator (PMI) agar and 55°C incubation, and (d) PMI agar and 64°C incubation. A β-lactam concentration of 0.008 IU/ml was detectable 100% of the time by variations a, b, and c and 98% of the time by variation d, with average zone diameters of 18.7, 17.7, 16.4 and 17.6 mm, respectively. The 0.005 IU/ml concentration was detected 92% of the time by variation a, 99% by b, 57% by c and 89% by d, with average zone diameters of positive units of 16.3, 15.5, 14.9 and 15.2 mm, respectively.This publication has 3 references indexed in Scilit:
- Bacillus stearothermophilus Disc Assay for Detection of Inhibitors in Milk: Collaborative StudyJournal of AOAC INTERNATIONAL, 1982
- A Collaborative Study of the Spiral Plate Method for Examining Milk SamplesJournal of Food Protection, 1977
- Spiral plate count method for the examination of raw and pasteurized milkApplied and Environmental Microbiology, 1976