Expression of Green Fluorescent Protein as a Marker for Effects of Antileishmanial Compounds In Vitro
Open Access
- 1 December 2001
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 45 (12) , 3654-3656
- https://doi.org/10.1128/aac.45.12.3654-3656.2001
Abstract
TransgenicLeishmania infantumpromastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.Keywords
This publication has 30 references indexed in Scilit:
- Nuclear Envelope Fission Is Linked to Cytokinesis in Budding YeastExperimental Cell Research, 2000
- Miltefosine, an Oral Agent, for the Treatment of Indian Visceral LeishmaniasisNew England Journal of Medicine, 1999
- Combination Allopurinol and Antimony Treatment versus Antimony Alone and Allopurinol Alone in the Treatment of Canine Leishmaniasis (96 Cases)Journal of Veterinary Internal Medicine, 1999
- Green fluorescent protein (GFP): applications in cell-based assays for drug discoveryDrug Discovery Today, 1999
- Clinical, Serologic, and Parasitologic Follow‐Up after Long‐Term Allopurinol Therapy of Dogs Naturally Infected with Leishmania infantumJournal of Veterinary Internal Medicine, 1999
- Combination Allopurinol and Antimony Treatment versus Antimony Alone and Allopurinol Alone in the Treatment of Canine Leishmaniasis (96 Cases)Journal of Veterinary Internal Medicine, 1999
- Generation of Destabilized Green Fluorescent Protein as a Transcription ReporterJournal of Biological Chemistry, 1998
- Microfilament dynamics during cell movement and chemotaxis monitored using a GFP–actin fusion proteinCurrent Biology, 1997
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- Mechanisms of drug resistance in LeishmaniaParasitology Today, 1993