Influence of the second and third codon on the expression of recombinant hirudin in E. coli
- 20 August 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 269 (1) , 244-246
- https://doi.org/10.1016/0014-5793(90)81164-j
Abstract
The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product accumulation follows the introduction for ile of the ATA codon which is of very low usage, and for thr of the ACC codon, which results in homology of the mRNA with the 3'-end of 16S rRNA. The results are discussed according to current concepts of protein expression in E. coli.Keywords
This publication has 10 references indexed in Scilit:
- Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiaeBiochemistry, 1989
- Messenger RNA recognition in Escherichia coli: a possible second site of interaction with 16S ribosomal RNA.The EMBO Journal, 1988
- Model for the three-dimensional folding of 16 S ribosomal RNAJournal of Molecular Biology, 1988
- RNA structural elements for expression in Escherichia coliFEBS Letters, 1986
- Effect of distribution of unfavourable codons on the maximum rate of gene expression by an heterologous organismJournal of Theoretical Biology, 1986
- Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.Proceedings of the National Academy of Sciences, 1986
- Characterization of translational initiation sites inE. coliNucleic Acids Research, 1982
- Codon catalog usage is a genome strategy modulated for gene expressivityNucleic Acids Research, 1981
- Improved Estimation of Secondary Structure in Ribonucleic AcidsNature New Biology, 1973