Phage F0lac: An F0lac Plasmid-dependent Bacteriophage

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Abstract
By enriching sewage with Escherichia coli or Salmonella typhimurium strains harboring the plasmid EDP208, a constitutive pilus-producing derivative of plasmid Folac, a phage was isolated which plated on these 2 organisms but not on isogenic strains without the plasmid. The phage was named Folac; it had a hexagonal shape with a diameter of 28 nm, contained RNA, was resistant to chloroform and probably adsorbed preferentially to the sides of EDP208 pili very near the tip. Phage multiplication could be demonstrated on E. coli or S. typhimurium strains carrying the plasmid Folac, but an increase in titer did not occur on E. coli strains carrying plasmids of the F complex. Phage multiplication experiments on strains carrying the depressed pilus-producing plasmids R71 or TP224-Tc, which determine pili serologically related to those of EDP208, were inconclusive. Phage Folac was found to be serologically related to phage UA-6, another isolate specific for EDP208.