Survival of Largemouth Bass Iridovirus in Frozen Fish

Abstract
Juvenile largemouth bass Micropterus salmoides were infected with largemouth bass iridovirus by injection and frozen at −10°C, and the visceral organs and tissues (liver, kidney, spleen, gonads, swim bladder, and muscle) of individual fish were periodically assayed for virus for 155 d postfreezing. The virus was isolated from 35 of 39 (89.7%) of the frozen fish. However, virus loss occurred more rapidly in fish that were dead when frozen than in fish that were alive when frozen. Overall, largemouth bass iridovirus was stable in frozen tissue when the fish were fresh upon freezing.

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