Glucose Metabolism of Tumors Induced by Rous Sarcoma Virus. I. Comparison of Chorioallantoic Membrane With Tumor of the Chorioallantois Induced by Rous Sarcoma Virus2

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Abstract
Essentially linear rates of glucose utilization were observed over various concentrations (a) Tumor of the chorio-allantois induced by Rous sarcoma virus (RSV tumor) had a maximal rate of glucose utilization 6 times that of chorioallantoic membrane (CAM). (b) Half maximal velocity was achieved with 5 mmoles per liter glucose by RSV tumor and 1.3 mmoles per liter glucose by CAM. Glycolytic activity was markedly greater with RSV tumor than CAM under aerobic conditions. Under anaerobic conditions the difference was not as pronounced: (a) Lactate accounted for 50 to 100 percent of C14 glucose metabolized by RSV tumor under both aerobic and anaerobic conditions. (b) About 25 percent of the glucose metabolized by CAM aerobically was recovered as lactate; anaerobically, 30 to 80 percent. With lower concentrations of glucose a greater percentage could be considered lactate from both tissues. Only 5 to 6 percent of the glucose used by RSV tumor was recovered as CO2, while there was 50 to 60 percent from CAM. About 5 percent of the glucose used by RSV tumor was recovered in fat. This was 10 to 20 times the amount recovered as glycogen. Very little C14 glucose was recovered as glycogen or fatty acids in CAM. Lactate selectively suppressed oxygen uptake of RSV tumor. Proposed mechanisms of the Crabtree effect are discussed with regard to the data presented.

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