Selective Simultaneous Proton Magnetic Resonance Labelling of Hydrophilic and Hydrophobic Regions of Phospholipid Vesicles. Effect of Polar and Apolar Reagents

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Abstract
Methyl-12-dimethylsilastearate was synthesized and incorporated by sonication into lecithin vesicles. In the resulting mixed vesicle the nuclear magnetic resonance line width of the Si-methyl resonance line is proportional to the lecithin–silastearate ratio, indicating interaction between the hydrocarbon chains of the phospholipid and the labelled fatty acid ester reporter molecule. The Si-methyl line reports selectively perturbations of the structure of hydrophobic regions as shown by addition of hydrocarbons. The N-methyl resonance line of lecithin is not affected by these changes, but indicates selectively effects on the hydrophilic exterior of the micelle produced by salts. The mixed vesicle may therefore be used as a membrane model allowing simultaneous observation of effects on the exterior and interior of the lipid bilayer.The silastearate label was successfully incorporated into Acholeplasma laidlawii membrane, which however failed to give a sufficiently resolved nuclear magnetic resonance spectrum.

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