DNA Sequence Homology between Autographa californica and Orgyia pseudotsugata Nuclear Polyhedrosis Viruses

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Abstract
To investigate the DNA sequence homology between the multicapsid nuclear polyhedrosis virus of A. californica and the multicapsid and single capsid nuclear polyhedrosis virus of O. pseudotsugata, the stringency of hybridization conditions was varied. Homology (13-25%) between the multicapsid viruses of A. californica and O. pseudotsugata was detected in 20, 30 and 40% formamide, indicating that fairly stable duplexes were being formed. Under the most stringent conditions in 50% formamide little homology was detected. In contrast to the multicapsid viruses, the single capsid virus of O. pseudotsugata demonstrated .apprx. 10% sequence homology in 20% formamide, but these duplexes were unstable and the percentage homology decreased markedly in the higher formamide concentrations. Examination of the specific regions of homology by hybridizing labeled DNA to Southern blots of the virus DNA revealed that the regions of homology between these viruses were not limited to one region of the genome but were found on a number of restriction fragments.